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This is a compilation of successful protocols that have been used by either our laboratory or other laboratories at Hopkins that have generated gene targeted mice.

We would like to thank Mitra Cowan, Mr. M. Dehoff, Dr. Kogo Takamiya, Dr. Yanshu Wang, Dr. J. Yang, and Ms. J. Wojcik for contributing protocols and advice on developing ES cell screening and culture systems.

ES Cell Culture

MEF Cell Culture

DNA Preparation for Testing

Reagent Information

Timeline Overview For:



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